Quantitative Detection of Schistosoma japonicum Cercariae in Water by Real-Time PCR
نویسندگان
چکیده
منابع مشابه
Quantitative Detection of Schistosoma japonicum Cercariae in Water by Real-Time PCR
In China alone, an estimated 30 million people are at risk of schistosomiasis, caused by the Schistosoma japonicum parasite. Disease has re-emerged in several regions that had previously attained transmission control, reinforcing the need for active surveillance. The environmental stage of the parasite is known to exhibit high spatial and temporal variability, and current detection techniques r...
متن کاملField detection of Schistosoma japonicum cercariae in environmental water samples by quantitative PCR.
A species-specific quantitative PCR (qPCR) assay was combined with two novel water-sampling methods and compared with the mouse bioassay for the quantitative detection of S. japonicum in surface waters. The novel methods were capable of capturing cercariae and, with subsequent analysis through qPCR, detecting the presence of a minimum of 1 cercaria.
متن کاملPCR detection of Schistosoma japonicum cercariae: a potential tool for the field
Despite decades of prevention and control efforts, the water-borne parasitic disease schistosomiasis remains endemic in 74 of the world’s developing nations. Five species of the Schistosoma trematode are pathogenic to humans; S. japonicum is the infective agent of schistosomiasis in China and the Philippines. The purpose of this research is to develop a sensitive and specific technique for the ...
متن کاملDetection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay
Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples wer...
متن کاملNovel real-time PCr for detection of Schistosoma japonicum in stool.
Chemotherapy has been used on a large scale in countries where the blood fluke Schistosoma japonicum is endemic. This has led to a lower intensity of infections and consequently lower diagnostic values of commonly used diagnostic tests like serology and Kato-Katz stool smear. We designed a novel real-time PCR method for detection of S. japonicum in stool samples. Further, we evaluated different...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLoS Neglected Tropical Diseases
سال: 2008
ISSN: 1935-2735
DOI: 10.1371/journal.pntd.0000337